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Questions related from Aman Ullah
Hello I am doing multiplex PCR for SCCmec typing of MRSA, but I am not able to get a band which is 1791bp and it is the largest band while all other five bands are present as intended. I used DNA...
13 August 2024 1,198 7 View
I have prepare 10micromolar solution of each primer, but I would like to prepare a Primer-Mix of these primers for M-PCR. How much volume of each primer need to added to create a primer mixture...
24 June 2024 2,249 3 View
I am extraction DNA from MRSA through a procedure in which I need to add the proteinase K and 0.1 M tris buffer simultaneously, so can I prepare the solution of PK in tris buffer and store in...
17 January 2019 6,519 2 View
Dear all, Anyone would like to share his/her opinion about the following 1. Can we add direct colony from selective media by harvesting in glycerol and broth mixture for preservation 2. Is there...
13 August 2018 1,792 2 View
I need to conduct some of PhD research experiment in Denmark but I want to know about the legislation of transportation of Clinical Isolates of MRSA from Pakistan to Denmark.
23 April 2018 3,297 2 View
I am searching literature for finding standard set of primer for mecA detection in MRSA but I am confused because different people use different set and every one claim it significant. Now I need...
08 February 2018 2,409 3 View
Can I use directly colony for agar plate instead of broth culture if no then which broth is more suitable
28 October 2017 2,650 5 View
Research in TB Is there a reference for the quote that Gene Xpert detect 131cfu/ml while LJ culture detect 100cfu/ml? Would anyone like to help me out ?
18 November 2015 5,762 0 View
