Amalia Kotsifaki

3 Questions 3 Answers 0 Followers

Questions related from Amalia Kotsifaki

How long can I preserve tissue at 4°C before initiating primary culture, and which media should I use for preservation?

Hello everyone, I am currently working on a project involving primary cultures of breast cancer tissue samples, and I have a few questions regarding preservation: How long can I safely preserve...

19 October 2024 9,653 1 View

What is the most effective protocol for extracting cells from breast cancer tissues? Which enzyme is considered ideal for this purpose?

I am currently conducting my PhD research aimed at isolating and culturing cells from breast cancer tissues for further molecular and cellular analyses. Effective cell extraction from tissue...

12 April 2024 4,424 4 View

Which is the Lipofectamine Transfection Protocol for plasmid DNA (PC3 Eb KO)?

Lipofectamine is a commonly used transfection reagent known for its efficiency in delivering nucleic acids into mammalian cells. However, the optimal transfection conditions can vary depending on...

12 April 2024 5,966 2 View