Álvaro Pascual

2 Questions 1 Answers 0 Followers

Questions related from Álvaro Pascual

Hybridization (FISH) on FACS sorted cells?

Hello, I am trying to perform a FISH with painting probes on a cell population selected by FACS but there are problems with the hybridization. The cells are fixed and detected by an indirect...

27 February 2023 8,981 0 View

How much volume for blocking and incubating antibodies in a cell sorting protocol?

I would like to know how much volume of a blocking solution and the volume of antibodies solution works best for a cell suspension. We resuspend the cells in 500 µL of blocking solution and then...

25 March 2022 814 1 View