Allison Anda

3 Questions 3 Answers 0 Followers

Questions related from Allison Anda

How to obtain empty vector from plasmid?

I require the empty vector of this plasmid: https://www.addgene.org/40346/ The ends have been destroyed: 5′ cloning site XhoI/SalI (destroyed during cloning) 3′ cloning site SalI/XhoI...

13 December 2023 1,174 4 View

How to calculate MFI comparing bimodal and normal distributed curves?

Dear researchers, A surface marker presents as a bimodal peak, however upon treatment the marker is a singular, wider spread peak. How would I calculate a comparable MFI between the two...

04 December 2023 7,372 0 View

How do you find IC50 for a 7-day treatment?

I want to perform a 7-day treatment (with replenishment of medium at day 3). I will be using the IC50 value for my experiment. Which time-point for the IC50 value should I use? I was thinking...

02 April 2019 4,030 4 View