Alkisti Mikrogeorgiou

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Questions related from Alkisti Mikrogeorgiou

Problem with NeuN/GFAP/DAPI triple staining in cortical neuron cultures

I have attempted staining primary cortical neurons from mouse in order to identify the percentage of astrocytes. I used GFAP rabbit (Z0334) from Dako and NeuN mouse (MAB377/X) from Milipore, both...

17 July 2017 6,263 3 View

Ideas for staining mouse brain sections cut in vibratome at the chronic phase of brain injury?

I am planning to stain floating sections of mouse brains that were fixed in 4% PFA and then kept in PBS. The mice had undergone hypoxic ischemic brain injury as neonates, and sacrificed as adults...

06 July 2017 1,314 3 View

Protocol for RNA immunoprecipitation in nuclear lysates from neurons

Does anyone have a good protocol for RNA immunoprecipitation in primary cortical neurons? I am specifically wondering what would be an ideal cell number to create nuclear lysates and perform RIP....

01 January 1970 7,740 0 View