Aline D. de Lima

5 Questions 3 Answers 0 Followers

Questions related from Aline D. de Lima

Kit ELISA or WB for characterization of murine extracellular vesicles?

Dear all, I'm looking for the best method for characterization of murine extracellular vesicles. It seems that the PS CaptureTM Exosome ELISA Kit (Streptavidin HRP) works well for humain EV but I...

24 January 2022 6,057 0 View

For T Cell Proliferation Assay I need to add a cell isolation step?

Dear all, To observe the immunosuppressive function of Treg, I will perform two different approaches of T cell proliferation assay. 1. 1.10^5 Splenocytes from mice labeling with cell trace violet...

23 November 2021 6,899 5 View

MLR - Co-culture Mouse splenocytes and PBMC human?

Dear all, I started performing Mixed Lymphocyte Reaction (MLR) to observe the immunomodulatory effects of regulatory T cells. The protocol stimulates the lymphocytes from the mouse spleen using...

28 April 2021 5,041 1 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

24 February 2021 5,760 4 View

It is possible isolate PBMC from mice for macrophage polarization assay?

Hello everyone, I am looking for an established protocol to macrophage polarization using PBMC from mice. But I don't know the density and proportion of monocytes after PBMC isolation. All the...

01 February 2021 5,002 2 View