Alina Makeeva

2 Questions 2 Answers 0 Followers

Questions related from Alina Makeeva

Should I order necessarily azide free primary antibodies for IHC, if I have HRP conjugated secondary antibodies?

I was wondering if the amount of sodium azide in primary antibodies for IHC can affect HRP activity in secondary antibodies, as I wash slides several times between the primary and the secondary...

13 September 2023 9,464 1 View

Does anybody have a good working protocol for IHC (tryptase) in mast cells?

I am a medical doctor and I do not have any experience in IHC, only toluidine blue and HE staining. Can you please share a protocol with me? Thank you in advance

13 September 2023 7,596 2 View