Alina Makeeva

4 Questions 2 Answers 0 Followers

Questions related from Alina Makeeva

Can BSA be substituted with FBS in the cell medium?

I am currently cultivating cord blood-derived mast cells. May I use FBS instead of BSA? If yes, what will be the proportion for the substitution? Thanks a lot in advance for sharing your experience

16 October 2024 5,019 4 View

Should I order necessarily azide free primary antibodies for IHC, if I have HRP conjugated secondary antibodies?

I was wondering if the amount of sodium azide in primary antibodies for IHC can affect HRP activity in secondary antibodies, as I wash slides several times between the primary and the secondary...

13 September 2023 9,502 1 View

Does anybody have a good working protocol for IHC (tryptase) in mast cells?

I am a medical doctor and I do not have any experience in IHC, only toluidine blue and HE staining. Can you please share a protocol with me? Thank you in advance

13 September 2023 7,649 2 View

How to cultivate HMC-1.1 and MCPV-1.1 human mast cell lines?

I am currently working with different mast cell lines, two of them do not want to show viability higher than 75-80 % HMC-1.1 I cultivate with IMDM (without L-glu)+ 10% FBS+ 1% p/s+ 1.2 mM...

03 November 2022 5,284 0 View