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Questions related from Ali Kermani
Hello everyone, I'm comparing the structure of a potassium channel in association with two distinct auxiliary proteins (B-subunits). In order to compare the interface between the potassium...
28 July 2022 5,536 0 View
I recently solved a crystal structure of a drug exporter bound to methylviologen (paraquat). When I was placing the substrate into the binding pocket, I realized that the two phenyl rings can...
27 October 2021 7,639 1 View
If you have a new substrate that never been crystalized before, how would you create a corresponding cif file to refine it? One way was to use eLBOW from Phenix package by using the SMILES string...
27 October 2021 4,805 4 View
I'm working on a new class of transporters in bacteria. I optimized the protein yield to about 3 mg per liter of culture, therefore I can generate enough protein to play with. So far I have tried...
09 January 2017 340 4 View
Using size exclusion chromatography I have shown that two proteins from one pathway form a complex. How can I co-crystallize these two proteins together? Shall I purify them separately and then...
16 April 2013 5,384 4 View
