Ali Ghorbani

6 Questions 11 Answers 0 Followers

Questions related from Ali Ghorbani

Why when I add Bromophenol Blue directly to my master mix, My PCR is inhibited?

Hi everybody, I have tried to make my home-made master mix for our laboratory. I have used two type of dyes , cresol red and Bromophenol Blue(BPB). I see when I use BPB my PCR is inhibited but no...

11 May 2024 7,465 8 View

The good software or tool for TCR repertoire analysis (spectratyping) ?

Hey everybody, I am looking for an easy method and also software for analyzing TCR repertoires. is Spectratyping the easiest method? do you suggest any method which is easy to set it up in the...

25 April 2023 9,820 4 View

Extracted huamn genome is partialy digested after treating with digestin enzymes?

Hi everyone, I have extracted the human genome from whole blood and tried to digest 2 ugs of it with 20 units of Bcll enzyme. unfortunately, after some trials, I observed that most of the genome...

28 December 2022 7,079 2 View

Must protein be denatured for Western blotting?

Hi everybody, Im about doing western blot and I've read that proteins must be denaturated for Western blotting. I question how it can be possible for antibodies to detect my specific protein if it...

17 December 2022 6,413 5 View

Why sometimes human transcripts are exactly same with negative strand??

Hi guys, I don't know why when I try to blast a segment of a human transcript sequence ( from the ensemble) on a gene (from NCBI), it sometimes returns the negative strand. But it has to be...

19 August 2022 3,835 0 View

Why i cannot find SNPs frequency For some alleles in NCBI or Ensemble ??

Hi everybody, I'm looking for an SNP (rs497692), but when I insert the rs in the databases, it returns the alleles as T>A / T>C . But there is frequency for T and C . Not for A . Why? can...

15 August 2022 8,224 3 View