Alexandros Alexandratos

4 Questions 12 Answers 0 Followers

Questions related from Alexandros Alexandratos

DGGE bands enhancement?

My project concerns analysis of the microbial community of sediments from aquaculture systems in concrete tanks supplied with fresh sea water. We followed a molecular approach through PCR cloning...

07 July 2018 4,258 2 View

Rehydration buffer for 2D samples.

I am using the following rehydration buffer for my 2D samples. Do you think any of the components may be altered/ become unstable after long period of storage at -20oC and thus affect the...

12 December 2012 1,380 7 View

Does anyone have a reliable precipitation protocol for proteins prior to proteomics procedure?

I am looking for a precipitation protocol for protein samples before doing the iso-electric focusing (IEF). I have tried a TCA/Acetone protocol but the protein pellets I get at the end are very...

11 November 2012 6,676 6 View

Is it possible to freeze an antibody mix?

Is it possible to freeze a primary and secondary (Jackson ImmunoResearch) antibody mix several times and reuse it? Will the antibodies still retain their same affinity after a long period of time?...

10 October 2012 296 18 View