Alexandria Nichols

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Questions related from Alexandria Nichols

Issues with Human and Yeast Antibodies?

What is the common shelf life of human antibodies stored at -20C? (if anyone knows specifically the shelf life for human TDP1 ab that would be great). Also, our lab has been using both yeast and...

11 November 2017 5,022 1 View

Having issues with semi dry transfer using a 2 buffer system (Cathode and Anode BIORAD).

I have had issues with my larger proteins not transferring to the membrane. I use the BIORAD PVDF membrane and the gel is a SDS-PAGE gel. I usually run the transfer at 11V for one hour. Larger...

08 August 2017 7,554 1 View