Alexandra Gonzalez

3 Questions 1 Answers 0 Followers

Questions related from Alexandra Gonzalez

Can both MLPA and qPCR measure del17p, t(4;14) and t(14;16) from multiple myeloma tumors? Are there any other techniques for studying this 3 CA?

The International Myeloma Working Group proposed a risk classification for multiple myeloma based on FISH. For high risk is necessary at least one of the following: del17p, t(4;14), or...

16 October 2017 1,379 1 View

Some advise on PCR contamination? If I sequence my neg.control (seems contaminated) and I don´t find anything, can I trust my samples sequence?

I am doing a PCR from DNA extracted from blood (human) and bisulfite converted. It used to work fine until a month ago when the negative control (water + buffer+ Mg+dNTPs+ primers+ Taq platinum)...

26 January 2017 8,536 1 View

How can I interpret my DNA methylation results from Direct Bisulfite Sequencing?

I extracted DNA from blood and made bisulfite conversion. I did PCR (without cloning) and then sanger sequencing. I have an amplicon of 255 pb in a UTR gene. I also did PCR for the untreated DNA...

10 January 2017 5,384 4 View