7 Questions 11 Answers 0 Followers
Questions related from Alexander Schimming
Hi everyone! I am looking for CD20 positive cells. My specific question is, is there a possible database where to check for CD20 positive cell lines and where I can compare the amount of...
07 July 2013 1,354 2 View
My lab is experiencing unstable results with TF-1 cells and the CellTiter-Glo kit by Promega. It's a very easy assay, seeding different amount of cells, growing for 3 days, then measuring via...
02 February 2013 2,034 7 View
I'm facing some trouble with the dissociating of two antibodies. Short story behind it: I have patient sera with a complex of two antibodies. This complex I want to cut and use one of the...
02 February 2013 1,046 8 View
I'm trying to clean up an antibody from a serum sample. For this I have the Melon Gel Purification Kit. Has anyone ever used this one? So I worked my way through the protocol given by the kit....
01 January 2013 7,845 2 View
Has anyone experience with the "easyCyte 5" Flow Cytometer from Millipore "? I have a demonstration in a couple of days and I'm interested in researchers' experiences.
11 November 2012 913 12 View
Have any of you ever used the mycoplasma-detection kit by Invivogen based on the HEK-Blue-2 cell line and reagents? Can I compare its accuracy with a mycoplasma-PCR? If anyone has experience with...
11 November 2012 4,887 3 View
I am wondering about your blotting conditions in Western Blot (wet system). I am using the bio-rad system. Running my SDS-page of 30mA for about 1 hour and then blot the proteins on nitrocellulose...
06 June 2012 1,795 10 View
