Alexander Max Bornschlegl

2 Questions 4 Answers 0 Followers

Questions related from Alexander Max Bornschlegl

How exactly do SYTO13 and Ethidium Bromide(Live/Dead Stain) work together?

Is it possible to identify live cells with SYTO13 alone? Or does only staining in combination with Ethidium Bromide allow differentiation between live and dead cells? I am using a microscope that...

08 August 2019 8,159 1 View

In ICC, are there any negative outcomes from combining the blocking and primary antibody incubation steps together?

Here is my thinking: In theory, antibodies have higher affinity for their antigens/epitopes than the proteins in the serum used for blocking. Why not have them compete for the binding sight at the...

07 July 2019 5,605 6 View