Alexander Schimming

4 Questions 5 Answers 0 Followers

Questions related from Alexander Schimming

CD20 positive cell lines - where to check for the amount of expression?

Hi everyone! I am looking for CD20 positive cells. My specific question is, is there a possible database where to check for CD20 positive cell lines and where I can compare the amount of...

25 July 2013 5,867 2 View

Experience with Thermo Fisher Melon Gel IgG Purification Kit

I'm trying to clean up an antibody from a serum sample. For this I have the Melon Gel Purification Kit. Has anyone ever used this one? So I worked my way through the protocol given by the kit....

30 January 2013 10,039 2 View

Invivogen PlasmoTest (HEK-Blue-2 based)

Have any of you ever used the mycoplasma-detection kit by Invivogen based on the HEK-Blue-2 cell line and reagents? Can I compare its accuracy with a mycoplasma-PCR? If anyone has experience with...

15 November 2012 2,343 3 View

Western Blot conditions

I am wondering about your blotting conditions in Western Blot (wet system). I am using the bio-rad system. Running my SDS-page of 30mA for about 1 hour and then blot the proteins on nitrocellulose...

12 June 2012 7,630 9 View