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I'm doing an immunoprecipitation for a epitope-tagged protein expressed in mammalian cells (DH82- a dog cell line). I re-suspend my frozen cell pellet in lysis buffer (Tris-based with 100mM NaCl,...
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The virus I'm working with is a morbillivirus. I have approximately 50mL of cell culture media and want to get the virus as concentrated as possible to infect primary canine PBMCs. Thanks in...
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