3 Questions 1 Answers 0 Followers
Questions related from Alex Dimitri
I looked up a gene on Ensembl (https://useast.ensembl.org/index.html) to get its sequence. It states that the gene is located on the reverse strand. Is the sequence of nucleotide bases given 5' to...
12 December 2018 6,452 2 View
So I deleted an exon of a gene in a cell line using CRISPR. The region deleted is around 1500bp. I isolated colonies, extracted DNA and ran a PCR with primers designed upstream and downstream of...
12 December 2018 483 2 View
I've been running lots of gels in my lab and the ladder is usually stored in the -20 Freezer, but I left it out at RT on the bench overnight. Would this have any effect on its resolution/etc for...
10 October 2017 1,379 3 View
