Aleksej Drino

3 Questions 5 Answers 0 Followers

Questions related from Aleksej Drino

How to get rid of glycogen from RNA pellet?

I am performing some chromatography with RNA I precipitate using Ambion glycoblue. However, I see that the precipitant is hampering the column run by sequestering and changing the viscosity of the...

08 November 2017 5,923 0 View

Low yield of binding RNA on an Q sepharose column?

I am trying to separate and concentrate tRNAs from high molecular weight (HMW) RNAs on an Ion Exchange Q sepharose FF GE column. tRNAs should come down at around 500-600 mM NaCl, and they do....

01 February 2017 8,168 6 View

Why do I have a low yield attaching amino-modified oligonucleotides to a NHS solid support ?

Hi, guys. I am attaching a 5' aminohexyl-modified oligonucleotide to a NHS activated sepharose. I have re-suspended 180 ug of IDT aminohexyl oligo in 1 mL of binding buffer (0.2 M NaHCO3, 0.5 M...

08 December 2016 4,122 4 View