Aleksandra Somogyi

6 Questions 9 Answers 0 Followers

Questions related from Aleksandra Somogyi

What's the specificity/tolerance of TaqMan probes for differences in sequence?

Hi, Does my TaqMan probe recognize the gene only if the sequence has 100% coverage with the probe or will I also get signal if there is a SNP or even a few bp difference? And if a few bp are ok...

10 April 2024 6,837 3 View

Why is the Lamp1 band in all my Western blots around 80 kDa and not 44 or 120 kDa as expected?

These are cerebellar granule neuron precur cell lysates. Antibody used was ab24170 from Abcam. I always get one band around 80-90 kDa, but not as expected the 44 kDa band or the glycosylated 120...

01 August 2016 9,089 3 View

Is there an overview of typical cellular stainings?

Hi, I am looking for a homepage or something else that gives a broad overview how the stainings of typical cellular markers look in different cell lines in order to compare it with my own results....

02 March 2016 4,759 3 View

What is the counterpart for an aliphatic amino acid in the three letters code?

CaaX means in the one letter code cystein - two aliphatic amino acids - unknown amino acid. How would this look like in the three letter code?

06 November 2015 5,820 3 View

Are there differences in the binding affinity of filipin to free and accumulated cholesterol pools?

I was told that detection of not accumulated intracellular cholesterol can be problematic with filipin. Is there any paper on that topic?

28 July 2015 9,095 7 View

Why is Neuraminidase 1 not localizing to lysosomes in cerebellar granule cells?

I used an anti-Neu1 antibody in order to examine the localization of Neu1 in cerebellar granule precursor cells and wondered why it is not localizing to lysosomes as expected. So I used HeLa cells...

11 June 2015 7,402 4 View