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Questions related from Aleksandra Somogyi
Hi, Does my TaqMan probe recognize the gene only if the sequence has 100% coverage with the probe or will I also get signal if there is a SNP or even a few bp difference? And if a few bp are ok...
10 April 2024 6,839 3 View
These are cerebellar granule neuron precur cell lysates. Antibody used was ab24170 from Abcam. I always get one band around 80-90 kDa, but not as expected the 44 kDa band or the glycosylated 120...
01 August 2016 9,091 3 View
Hi, I am looking for a homepage or something else that gives a broad overview how the stainings of typical cellular markers look in different cell lines in order to compare it with my own results....
02 March 2016 4,761 3 View
CaaX means in the one letter code cystein - two aliphatic amino acids - unknown amino acid. How would this look like in the three letter code?
06 November 2015 5,821 3 View
I was told that detection of not accumulated intracellular cholesterol can be problematic with filipin. Is there any paper on that topic?
28 July 2015 9,096 7 View
I used an anti-Neu1 antibody in order to examine the localization of Neu1 in cerebellar granule precursor cells and wondered why it is not localizing to lysosomes as expected. So I used HeLa cells...
11 June 2015 7,403 4 View