Aleksandra S. Klosinska

2 Questions 1 Answers 0 Followers

Questions related from Aleksandra S. Klosinska

DOT BLOT - what do I resuspend precipitated proteoglycan for dot blot experiment?

I am digesting proteoglycans to test whether some antibodies can label my digest. However, once my protein has been digested and precipitated, I am not sure what is the best solution to resuspend...

20 June 2022 1,517 1 View

How to perform protein deglycosylation for WB and IHC?

Hello! I have samples of proteins extracted from aortic tissue using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents, I have attempted immunoblotting a few times already, but the bands I am...

10 November 2021 8,577 1 View