Can someone provide advice on how to make ligation work?

Vector Info: Pcdna (6500bp) 5ug of vector Digested with HINDIII & ECORI in cut smart for 3 hours at 37 ( with 1uL of CIP). Gel purified Insert info: (1800bp) PCR product digest with HINDIII...

02 February 2015 7,006 16 View