Alaa Youssef Farag

6 Questions 10 Answers 0 Followers

Questions related from Alaa Youssef Farag

How to optimize syber green RT-qPCR ?

I am very new to real time PCR (I did my first run today) and i got nothing so i will really appreciate your patience and detailed answers. As a beginning i wanted to test the expression of one...

01 January 2019 5,926 4 View

I am getting no amplification curve at all and weird melting peaks?

I would really appreciate your support as this is my first introduction to the real time PCR. I am performing relative gene expression using power up syber green and on a biorad cfx instrument....

01 January 2019 8,289 6 View

MiRCURY mimics and inhibitors or miscrip mimics and inhibitorst are the best for miRNA transfection?

Hi All, I am going to transfect a specific miRNA into snu449 and hepG2 cells. I am still confused about which to buy as qiagen has two different lines of mimics and inhibitors, miRCURY line or...

10 October 2018 2,240 3 View

MiRCURY LNA assays from Qiagen and Syber green master mix from Applied biosystems ?

Hi guys, i am going to test the expression level of a specific miRNA in some cell lines using Real time PCR. I am also intending to test the expression levels of some potential downstream targets...

10 October 2018 1,388 3 View

Buying mirNA mimic and inhibitors from qiagen ?

I want to run a transfection experiment using mimics and inhibitors of a specific miRNA. Since it is my first experience in the master's thesis, I don't know which companies are the best. in the...

09 September 2018 2,738 4 View

Taq-man universal master mix for miRNA in real time PCR?

I will do a real time PCR using taqman assay for a specific microRNA. I am very confused about the best master mix to use. I found that taq-man has 4 master mixes; 1-with no Ampearse UNG 2-with...

09 September 2018 3,456 3 View