Akshat Sharma

4 Questions 15 Answers 0 Followers

Questions related from Akshat Sharma

Picrosirius Red Staining from Frozen Liver Sections?

Hey, y'all! I am planning on staining mouse livers with picrosirius red, and I have found excellent protocols to do this and a sense that this absolutely can be done from frozen sections. The...

02 February 2019 577 1 View

What is (should be) the ratio of K562 transfectants to PBMC for NK cell expansion?

Hey, all!So, I am trying to expand NK cells from PBMC. It seems simple enough, but I am wondering what the ratio of PBMC: irradiated K562-41BBL-IL-15 should be. I did 0.5x10^6 PBMC: 1.5x10^6 K562...

03 March 2015 2,787 1 View

When performing an Adhesion Assay of T cells to ICAM-1 should I Block or Not Block with PBS-3%BSA?

I am currently optimising a plate-bound adhesion assay to study the adhesiveness of polyclonal human T-cells to differing concentrations of ICAM-1. I start with coating the plate with ICAM-1-Fc....

01 January 2015 4,211 14 View

What is the cell density for fluorimetry/adhesion assay?

I'm developing an adhesion assay with CFSE-labeled polyclonal T-cells. Basically, I allow the cells to adhere to a poly-l-lysine coated (0.01%) well in a 24 well plate, and after gentle washing, I...

09 September 2013 5,466 7 View