Ajit Singh

7 Questions 22 Answers 0 Followers

Questions related from Ajit Singh

Whom should I approach for analysis and assembly of NGS reads of nanobodies in the camel phage display library?

We constructed Nb-phage display library derived from an LPS-immunized Indian desert camel and have recently subjected it to NGS using Illumina MiSeqTM platform. The raw data of NGS reads was...

25 May 2020 8,280 3 View

Has anyone guesstimated the impact on microbiomes due to large scale global use of sanitizers and disinfectants in the wake of Covid-19 pandemic?

Microbiomes are affected by disinfectants and sanitizers. Excessive use can reduce the density of 'friendly microbes' in biotic and abiotic ecosystems. The dilemma is: in fighting against one, we...

18 March 2020 2,134 9 View

Has anyone estimated the number and catalogued the extinct virus genera or species?

Viruses are obligate intracellular parasites of diverse hosts ranging from prokaryotes to eukaryotes belonging to all phyla. Several host species have gone extinct at different times, so must have...

27 July 2017 10,205 5 View

Is it possible to precipitate a simple (single chain) protein antigen with a monoclonal antibody in solution?

A simple protein of about 55 kDa size can have several epitopes. If an epitope is repeated on its structure, a specific (bivalent) monoclonal antibody can interact with these epitopes to result in...

19 November 2015 8,337 2 View

What could be the possible ways of clearing endotoxin neutralized in vivo- without showing ill-effects?

Endotoxin can possibly be neutralized in vivo by diverse substances. The endotoxin- neutalizing substance complex must be removed from the body before it could cause CD14-TLR4-mediated activation...

10 November 2015 4,074 2 View

How to achieve repeatability of LAMP results?

Three sets of primers, various conc. of Mg2+, 1x amp buffer (NEB), Bst2.0 warm start (NEB), 0.8M betaine, 1.4 mM each dNTPs, 25 ul finalreaction vulme, 63C, 1h, followed by 80C, 5 min. Sometimes...

11 February 2014 3,979 4 View

Any parasite that caused extinction of its host species ever?

25 June 2013 3,829 15 View