Ajit Kumar

6 Questions 10 Answers 0 Followers

Questions related from Ajit Kumar

Getting Problem in the syringe cleaning and completely Drying in the MicroCal PEAQ-ITC?

After washing the cell and syringe, I am facing a problem with the complete drying of the syringe. I am getting a large peak in the water-water run which might be due to residual methanol in the...

27 April 2023 6,594 1 View

Getting zig zag pattern in MicroCal PEAQ-ITC after saturation?

I am titration protein in the RNA sample. Titration was performed in binding buffer, RNA in the cell was 2.5uM and protein in the syringe was 96uM, with 19 injections (2uL, 4s. I have attached the...

20 May 2022 3,633 2 View

What could be the reason for no protein expression in a tet-on Anhydrotetracycline inducible system in M. smagmatis?

I have Cloned a Mycobacteraial gene in pST-KT Anhydrotetracycline(ATc) inducible vector and electroporated in M. smagmatis. Transformants grown till OD 0.6-0.7 and 200ng/ml ATc were added and...

01 August 2021 8,233 2 View

Smering in Pcr Amplification ?

I am facing a problem regarding smearing in PCR amplification since two months. But, before six months the results was there using the same primers at 67.2-degree celsius annealing temperature, 5%...

24 November 2020 4,545 5 View

Is there any online tool by which we can determine the length of N-terminal and C-terminal of the protein sequence?

I have our protein sequence and I knew the N and C terminus ends, but I want to know that from where the N terminus ends and the C terminal end has started.

08 September 2020 3,455 5 View

I am getting error saying could not open conf.txt for reading in AutoDock Vina.?

When I tried to docking windows cmd shows Error: could not open "conf.txt" for reading. I have tried to change the path for cmd e.c.t, but it was not helping. And I tried to change from program...

11 May 2020 9,496 4 View