Aisyah Jaafar

3 Questions 2 Answers 0 Followers

Questions related from Aisyah Jaafar

What are the recommended storage conditions for cell lysate prior to ELISA?

I am using a mammalian cell line (Vero) and need to infect the cells with a virus resulting in reduced cell number at the end of the experiment. I plan to standardise either the number of cells...

07 December 2022 3,604 2 View

How to prepare stock solution of Ascorbic Acid (AA) or Vitamin C for cell culture treatment?

I want to include AA/Vit. C as a positive control for my antioxidant study using Vero cells to compare with my drug compounds. I will measure MDA (lipid peroxidation marker), SOD, and Catalase...

27 July 2022 6,069 2 View

How to collect and freeze samples for colorimetric assays?

I will need to harvest different batches of cell samples at different time points. Then I will perform homogenization on a separate day to get the cell lysate and run the assay using all samples...

03 February 2022 2,228 0 View