Aishwarya Sen

3 Questions 3 Answers 0 Followers

Questions related from Aishwarya Sen

Why am I failing to split the freshly transduced mutuDC cells?

Necessary information- 1. Target cell line: mutuDC/ DC1940 (mouse CD8a+ immortalized cells). Passage is over 25 (early passage not available). Cells are cultured in IMDM media (10% FBS, PenStrep,...

05 December 2024 7,453 1 View

How do I go about with the sorting of bone marrow derived dendritic cells (BMDCs) without hampering their survivability?

Hello! I am having problem in sorting BMDCs from a heterogenous population of murine hematopoietic stem cell which have been cultured in RPMI+10% FBS+ 1% PenStrep with FLT3/L (for differentiation)...

23 March 2022 1,734 2 View

How do I go about with a Dendritic cell-T cell co-culture experiment?

Hi! I have cultured human CD34+ cord blood derived CD141+ dendritic cell (DC) using IL-4 and GM-CSF and have achieved around 30% CD141+ DCs. Now, I'm planning to stimulate these DCs with poly I:C...

04 January 2022 5,519 2 View