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Hello, I have a basic question. I work with mouse hearts and I want to collect them to do bulk RNA-seq in the future. What is the best way to store them? Right now it was recommended to collect...
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Hello. In our manuscript we analyzed immune cells in food allergic mice. As part of the review of the literature, we analyzed human RNA-seq samples that were available in GEO database. The...
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Hi! I just had a manuscript rejected for publication, reviewer 2 stated that since we did not perform repeats, just replicates, the reproducibility of the results is compromised. Basically, our...
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Hello. I am used to paraffin protocols, but now I need sections of mouse heart from frozen tissue due to not having paraffin equipment. My protocol is: Remove the heart from the animal and...
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