Ahmed Alfahdawi

4 Questions 6 Answers 0 Followers

Questions related from Ahmed Alfahdawi

Matrigel Corning 356230 (GFR) stock concentration ?

Hi everyone I have recently purchased Matrigel corning 356230 (GFR) to perform cell invasion assay, does anyone know the stock concentration so I can dilute it please. I have looked through the...

01 January 2020 8,758 1 View

Do normal fibroblast turn into CAFs when exposing them to cancer cells ?

I am tending to use CAFs in our lab but I do not have them, so I am thinking to expose them to breast cancer cells in order to turn them into CAFs. Any idea please ?

03 March 2019 8,864 3 View

Does anyone use macrophages derived from Thp-1 cell line instead of primary macrophages ?

In my PhD, I am trying to evaluate the role of macrophages in breast cancer, my supervisor suggested to use macrophages differentiated from Thp1 with PMA, instead of using primary macrophages....

03 March 2019 2,159 4 View

Triple cell culture protocol

Hi there, I am tending to do triple culture (Epithelial cells, fibroblasts and macrophages in order to test cells migration, invasion and proliferation in 96 well plate. Can anyone tells me how...

01 January 1970 9,663 4 View