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I am planning to do skin FACS for mouse, and was wondering if any has tried doing intracellular staining ( using pma ionomycin). What was the best concentration of pma ionomycin to use and would...
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I am trying to extract RNA from mouse skin reserved in Trizol. I tried the usual protocol, it worked for all the other organs except the skin. It looks it is easily degraded. Does any of you know...
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What are the best markers for neutrophils in mice ?
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I am checking mice nephritis samples and want to adapt a kind of a scoring system to compare the degree of nephritis with time. Do you know any scoring system I can adapt ?
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