Ahmed Abdelhamid

4 Questions 4 Answers 0 Followers

Questions related from Ahmed Abdelhamid

Is there any professional cDNA synthesis tips for junior researchers ?

I am about to start cDNA synthesis from RNA as well as many researchers here. What tips you provide considering cDNA synthesis; which RNA samples to be used, kit, protocol, volumes, and...

29 October 2021 8,837 3 View

Spinning time of centrifuge ?

the protocol i am using for RNA extraction states i should spin at 12000 rpm for 1 min at some steps, however, the centrifugation device i am using reaches that speed after 1 min of hitting the...

03 October 2021 6,264 4 View

Low A260/280 ratio, RNA extraction ?

Iam using TRIazol-chloroform method for RNA extraction from PBMCs. However, the quality of isolated RNA is poor in some samples considering A260/280 ratios. are these samples valid again for...

01 October 2021 5,587 2 View

cDNA tips for junior researchers

I am about to start cDNA synthesis from RNA as well as many researchers here. What tips you provide considering cDNA synthesis; which RNA samples to be used, kit, protocol, volumes, and...

01 January 1970 2,378 3 View