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I am about to start cDNA synthesis from RNA as well as many researchers here. What tips you provide considering cDNA synthesis; which RNA samples to be used, kit, protocol, volumes, and...
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the protocol i am using for RNA extraction states i should spin at 12000 rpm for 1 min at some steps, however, the centrifugation device i am using reaches that speed after 1 min of hitting the...
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Iam using TRIazol-chloroform method for RNA extraction from PBMCs. However, the quality of isolated RNA is poor in some samples considering A260/280 ratios. are these samples valid again for...
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