Ah reum Kim

5 Questions 3 Answers 0 Followers

Questions related from Ah reum Kim

How long can I store a HPLC loading sample (protein) after buffer exchange?

i did exchange 0.1% TFA buffer in water. and protein sample is plasma. Before start experiment, i want to know my sample stability in TFA buffer. also, storage temperature... Thank in advance.

01 June 2017 3,997 1 View

Can i use Trizma base(sigma T6066) for HPLC start buffer?

I have Trizma base ( BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9%...

17 May 2017 9,370 3 View

How can i separate HSA(Human serum albumin) from Human plasma?

I want to separate HSA from human plasma... after then, i want purify albumin Using HPLC. But i don't know How can i separate HSA. I think i have to remove low abundant plasma protein before...

21 April 2017 4,321 7 View

Is it necessary add protease inhibitor before doing ELISA?

Hello. I wondering i must do add protease inhibitor(like a PhoSTOP,,) before ELISA experiment?... My sample is human plasma. Before start ELISA experiment, plasma samples are distributed 30ul,...

03 February 2017 2,958 3 View

I don't know what is different between two protein IDs at UniProt...?

Hello. in Uniprot site, i don't know what is different between D6RAR4 and Q04756. those proteins "Hepatocyte growth factor activator" But i don't know exactly what is different.... Please tell me...

03 January 2017 9,778 3 View