Agnieszka Kaczmarek

2 Questions 4 Answers 0 Followers

Questions related from Agnieszka Kaczmarek

Why do I observe nedcrosis while staining MDMs with cell tracker green?

Hi, I am culturing monocyte derived macrophages from human blood. For my assay I need to stain MDMs with Cell tracker green. Recently I get toxicity- cells literary turn secondary necrotic while...

06 June 2015 5,535 2 View

How can I analyse fluorescent images of Double/triple positive cells?

Hi I am using a Columbus for fluo images analysis. I need to define double or triple positive cells. My nuclei are stained with Hoestch (all cells), Sytox (dead cells only) and FITCs (apoptotic...

01 January 2015 4,177 5 View