Agnieszka Borys

4 Questions 8 Answers 0 Followers

Questions related from Agnieszka Borys

Why am I having huge shifts in reference genes Cq's?

I was recently performing a great deal of qPCRs on adipose tissue derived RNA. I had experimental samples from sick, and control samples from healthy individuals. I used the same chemistry for all...

05 June 2024 6,973 0 View

How can I easilly, quickly and open source compare NGS variant calling results and Sanger sequencing validation?

Hello RG, I have results from Sanger sequencing for validation of multiple variants I have found in my patients cohort, can you please instruct me if there is some sort of open source program...

22 August 2022 1,319 0 View

What happens with my PBMC's?

I'm trying to culture PBMC's isolated from whole blood. I'm using Histopaque 1077 and AccuSpin tubes. From around 10ml of WB I get up to 20mln of viable (V>90%) cells. I plate them in RPMI1640...

08 July 2014 5,858 12 View

Does anyone know about an ELISA kit that would measure small amounts of human urocortin 2 (UCN2) in serum/plasma?

Detection range around 10-20pg/ml. I have used one but it didn't even detect UCN2 added to the wells. Thanks.

21 May 2014 7,113 5 View