Agnes Liu

4 Questions 1 Answers 0 Followers

Questions related from Agnes Liu

Which one is more closely related?

Based on the phylogenetic tree attached, is Bacillus tequilensis more closely related to Bacillus safensis or Halalkalibacterium halodurans? Please let me know why too.

04 May 2025 7,096 1 View

Which Sequence to Use When Constructing a Phylogenetic Tree?

I want to construct a phylogenetic tree using the maximum likelihood method and the phylogenetic tree will be constructed based on the gene. But I am not sure whether to use the DNA sequences or...

25 April 2025 9,439 2 View

When constructing phylogenetic tree which is based on the rocF gene, do the reference sequences need to have the rocF gene too or can they be random?

I just finished sequencing my samples. Of the 11 samples, 7 isolates showed decent results after further analysis using SeqTrace. Afterwards, the sequences were uploaded to BLASTX to make sure...

02 April 2025 5,820 1 View

Is this electrophoresis result of 16S rRNA PCR proper for sequencing?

After doing bacterial genomic DNA isolation, I continue using the DNAs obtained for further 16S rRNA PCR. However, after reviewing the result, I notice there's a super faint band appearing in the...

28 January 2025 6,977 3 View