Adrienne Anderson

6 Questions 11 Answers 0 Followers

Questions related from Adrienne Anderson

Why are my negative control slides staining positive with IF?

Hello, I'm having some trouble with my negative control slides when I do immunofluorescence. I only work with frozen muscle tissue, and I'm trying to optimize a number of antibodies. For some of...

08 December 2016 3,539 7 View

Help with bovine muscle histology?

Hello, I am freezing "normal" bovine muscle (literally it is steak I got from the grocery store), and the pathologist is telling me that there is freezing artifact. Looking at an H&E, there is...

06 December 2016 331 10 View

Can I use IgG secondary with an IgG1 primary?

I just ordered a mouse monoclonal IgG1 primary antibody, and the only secondary I have is a goat anti-mouse IgG. Is this not specific enough? Do I need to have a secondary specific for IgG1? Thanks!

01 December 2016 6,403 4 View

How do I get rid of background staining in IF?

Hello, I'm having a lot of problems trying a paladin IF stain on skeletal muscle tissue. My negative controls seem to show more staining than my slides. For the negative control, I'm just omitting...

11 November 2016 8,291 2 View

How do you stain cultured cells on coverslips?

My colleague grew some cells on coverslips for me to do FISH and IF staining. This is my first time staining this way. Can anyone share their method with me? What I mean specifically are the...

31 October 2016 8,697 4 View

Fluorescence staining only the periphery of tissue?

I've been working on some FISH/IHC combo stains which have been working well until this last run where I got bright staining on the edge of the tissues but very minimal staining in the middle. The...

01 September 2016 10,012 5 View