Adrian Leung

4 Questions 2 Answers 0 Followers

Questions related from Adrian Leung

CHIP-qPCR high background in negative region?

I'm trying to do CHIP-qPCR on various cells lines on both histone markers and TF. The problem I've encountered is both high background in non-binding intergenic regions and also high binding in...

23 March 2023 9,302 0 View

Tamoxifen intragastric injection in perinatal mice?

Hi, I would like to inject tamoxifen to mice pups from P7 to P11 once daily. I’ve tried injecting 30ul of tamoxifen with 27G needle to intraperitoneal space but most leaked out. I came across an...

24 January 2023 7,768 1 View

Why is my GOI not expressing after lentiviral transduction despite RNA increase and protein is detected in transfection?

Hi, I'm trying to create a stable overexpression line with EF1a-FlagGOI-IRES-GFP using lentivirus packaged with pxPAX2 and pM2G. After transduction of 293T with 8ug/ml polybrene, I was able to...

30 January 2021 2,052 4 View

Uneven poly-d-lysine coating/crystallization?

I've encountered problem with large but scarce PDL crystals and spotted coating after 1hr of 0.02mg/ml PDL in PBS incubation, PBS washed and dried. Previous coating from same PDL stock resulted in...

05 November 2019 3,457 2 View