Adrian Ionut Cepleanu-Pascu

3 Questions 10 Answers 0 Followers

Questions related from Adrian Ionut Cepleanu-Pascu

What do you think this big smear is in lane 1?

So I have tried to PCR a fragment for my assemblies. (not so successful as of late I might add) although I have done them almost 100 times. I have changed polymerases (not related to my...

12 August 2023 7,889 0 View

Are S/MARS vectors maintained without initial selection?

Hello, Are any of you working on episomal MARS vectors? I want to know how much time / generations they last WITHOUT an initial selection in mammalian cells (any type would do). I could clone...

20 November 2021 897 0 View

Why do I get a smaller band than I should during PCR?

So I did a PCR today. I've tried to amplify a quite CG rich region from a plasmid pTriex1.1. My primers should anneal and form a 666 band, instead I get a band that migrates at about 300 - 400 kb...

18 August 2021 9,085 11 View