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Hi, I am looking for someone who is using the Soumpasis equation for fitting FRAP curves. Per se it is used for fitting one component fluorescent recovery curves, but i need to fit two...
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After adding ANS, I see a 5-6 fold increase in intensity but no blue shift in emission maxima. Could this be from scattering? Or is this also evidence of binding of ANS to hydrophobic patches?
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