Adi Lahiani

2 Questions 1 Answers 0 Followers

Questions related from Adi Lahiani

I want to quantify exosomes using BCA, can i use the final extracted exosomes or should i take sample from the CM before ultracentrifugation?

I want to quantify exosomes using BCA, can i use the final extracted exosomes or should i take sample from the CM before ultracentrifugation? How much ul of extracted exosomes do I need for BCA...

26 August 2021 1,241 3 View

If i forget to filter (0.2um) Condition Medium (collected for exosome isolation) before ultracentrifugation ?

I isolate exosomes from condition medium according to ultracentrifugation protocols. after 3 centrifugation (10 min x300g) then (15min x2000g) then (30 min x10,000g) , medium collected and filter...

12 August 2021 768 2 View