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Does anyone know what the biological function of hydroxypyruvate isomerase (Hyi) is in animal cells? I know that it catalyses the chemical reaction of hydroxypyruvate to 2-hydroxy-3-oxopropanoate,...
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I was wondering whether it is nescessary to crosslink DNA to a nylon membrane (for dot blot assay preparation), or is air-drying the DNA onto the membrane sufficient for strong binding prior to...
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Has anyone had any problems with their negative controls for some microRNA Taqman assays? With a few assays, replacing RNA with water for the RT reaction still results in qPCR amplification and a...
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