Adam K A Wright

4 Questions 3 Answers 0 Followers

Questions related from Adam K A Wright

Single Cell RNA-Seq PBMC 10X : Doublet Rate threshold?

Dear fellow scientists, When loading cells (human PBMCs) onto the 10X chromium controller there is a trade off between total cells loaded, recovery and theoretical doublet rate. I am using 2...

23 September 2021 855 0 View

Has anyone got any experience of using the LiveCyte Phasefocus with ibidi mu-chemotaxis slides to monitor monocyte migration?

I have access to a LiveCyte Phasefocus (without heat, CO2 capability) that has a slide adaptor able to take ibidi mu chemotaxis slides. I wish to monitor monocyte migration in real time using...

21 November 2019 214 3 View

Is Bambanker a good cryogenic solution for storing PBMCs in -80 and Liq N2?

We have recently utilised Bambanker (BB01) from Nippon Genetics (Geneflow in UK), which is a serum free DMSO containing cryogenic storage medium for cells. PBMCs (5x10e5/ml) were not happy in...

26 September 2018 2,209 3 View

Do human CD14++ monocytes also express CD34?

I have been using a CD34-PEcy7 reagent from Becton Dickinson (clone 581, class III epitope) to stain PBMCs for flow cytometric analysis. CD34 haematopoietic stem cells are not the only cells to...

31 March 2015 9,717 8 View