Adam Lin

2 Questions 2 Answers 0 Followers

Questions related from Adam Lin

How many DNA fragments should I use for the Gibson Assembly?

I have four DNA fragments for Gibson Assembly; two of the fragments are 5kb with a concentration of 0.011pmole/ul and 0.034pmole/ul, and the other two fragments are 0.5kb (0.163pmole/ul) and 0.6kb...

17 November 2023 5,850 0 View

CRISPR targeting in cell line, flow or single clone?

I am doing a CRISPR targeting to knock out one intron of my gene of interest in a cancer cell line and replace that intron with GFP. I am wondering if I should pick single cell clone with possible...

08 November 2023 2,942 0 View