Ada Tse

4 Questions 9 Answers 0 Followers

Questions related from Ada Tse

Why my HaCaT cells die after thawed?

I tried to thaw my HaCaT cells several times but 99% of them died. They were frozen with 10% DMSO + 10% FBS + 80% DMEM. I also tried 10% DMSO + 90% FBS then they died less but still only 10-20%...

19 October 2019 1,850 2 View

What temperature should I keep the blood before plasma preparation?

I need plasma samples for analysis of metabolites by NMR methods. I found protocols of plasma preparation suggesting keeping the blood at 4oC before centrifugation but I also found protocols...

02 April 2019 6,219 6 View

What is the best method of neutrophil isolation and anti-coagulant if I want 20 millions of healthy and unactivated cells in 95% purity?

I need at least 20millions of neutrophils from whole blood for metabolome analysis, so they must be unprimed, unactivated, viable and non-apoptotic. I tried MACSexpress but the purity is not high...

09 July 2018 5,222 11 View

Has anyone tried to add calcium chloride to calcium-free MEM to achieve 1.8mM calcium concentration? I tried but always have white precipitate. why?

I need to culture mammalian cells at low calcium 0.03mM and high calcium 1.8mM condition. There is only one suitable calcium-free medium available in the market (MEM, low glucose, no hepes). Then...

09 July 2018 8,646 1 View