Abrar Hussain

10 Questions 18 Answers 0 Followers

Questions related from Abrar Hussain

Hello dear experts, could someone please share a simple and easy protocol for "His-Tag Protein Purification"?

Dear experts, as I'm new and have no previous research experience related to protein purification, I tried different protocol but not get desire result. I am now working purifying protein via...

19 June 2025 734 4 View

What should I do in this case doing antioxidant assay for nanoparticle?

Respected expert, as I have done antioxidant assay for zinc oxide nanoparticle using medicinal plant Serriphidium kurramense. In this assay I have used Ascorbic acid as standard and DPPH as free...

24 November 2023 2,624 2 View

Why does the initial color change occur during the green synthesis of nanoparticles?

when we do synthesis of nanoparticle through Green route, we always notice the initial color change by adding Plant Extract and the Salt like Zinc acetate in ZnO NPs etc. and if the color change...

17 November 2023 1,803 2 View

How to get UV absorption peak of Green synthesized ZnO NPs mentioned in literature "350nm - 400nm"?

I have used different concentrations like I used 1/ 1mg ZnO NPs in 1ml DH2O, Peak "206nm 2/ 1mg ZnO NPs in 3m DH2Ol, peak "220nm" then I used 3/ 1mg ZnO NPs in 5ml DH2O , Peak "220nm" so what I do...

12 November 2023 9,811 2 View

How can I solve the given problem?

I have done the procedure many time for ZnO NPs synthesized via Green synthesis method from leaf extract but I didn't get the desire peak for UV absorption mentioned in literature that is from...

10 November 2023 2,717 0 View

How do I prepare a sample solution of Green synthesized ZnO nanoparticles present in powder form for UV-vis spectroscopy?

I have already used two concentrations, i,e: 1: Took 1mg ZnO NP in 1ml water 2: Took 1mg ZnO NP in 3ml water After taking these two concentrations, properly dispersed NP using Ultrasonic...

07 November 2023 9,898 3 View

Why black color powder (NPs) instead white color while synthesis Zinc oxide nanoparticles through greensynthesis method using the salt zinc nitrate?

As in different research papers in literature it is mentioned that you will get white powder means NPs as the end product but I have black color as the end product.

11 August 2023 5,588 1 View

Why i get control value(2ml methanol+1ml DPPH 0.1mM SOL) less then our sample ie, plant extract and Nanoparticles) in antioxidant assay?

I want to perform Antioxidant assay for Iron oxide NPs synthesized through green synthesis method from leaves extract and also wants to check the antioxidant capability the plant extract which is...

11 August 2023 3,717 5 View

Can someone give suggestions on Questionnaire based Research "Stress level comparison of Medical and Non-Medical Students" ?

I am starting Questionnaire based research on the the topic ""Stress level comparison of Medical and Non-Medical Students" so I need help because I have no previous experience of questionnaire...

21 April 2019 8,885 6 View

Antioxidant assay for zinc oxide nanoparticles

Respected expert, as I have done antioxidant assay for zinc oxide nanoparticle using medicinal plant Serriphidium kurramense. In this assay I have used Ascorbic acid as standard and DPPH as free...

01 January 1970 3,908 7 View