Abirami Santhanam

3 Questions 10 Answers 0 Followers

Questions related from Abirami Santhanam

How can I improve RNA yield from Laser capture microdissection (LMD)?

I am using rabbit cornea to do LMD and i am collecting the laser dissected fragments directly in the RNA lysis buffer as suggested in the RNeasy micro kit from qiagen where they have a protocol...

02 February 2015 4,829 8 View

Have any one used Bio Rad turbo transfer for transferring high molecular weight protein around 450kDa?

Hi, I am trying to use fast turbo transfer for transfer of my protein of mol.wt 450kDa. Is it possible to do by turbo transfer? I am trying to get the condition for perlecan western blots. Any...

12 December 2014 2,968 7 View

What is the best way to detect extra cellular proteins like perlecan and nidogen from cultured fibroblast using western blot?

Right now I am using 1% NP40 containing lysis buffer to collect the lysate from rabbit corneal fibroblast cells.

09 September 2014 8,279 5 View