Abhishek Chanda

3 Questions 2 Answers 0 Followers

Questions related from Abhishek Chanda

Has anyone observed double band for protein molecular marker in SDS-PAGE?

I have been observing double bands for protein molecular marker(especially 15 kDa band) every time I am doing SDS-PAGE. Sometimes even the samples appear to have a double band. These are 12.5%...

17 July 2023 2,184 4 View

How to dissolve a 21-mer synthetic peptide (acidic)?

I'm facing a problem in dissolving two synthetic peptides (21 amino acids long). The peptides have iso-electric points at pH 4.01 and good predicted solubility in water. However, the synthesized...

17 February 2020 6,760 3 View

Why does sediments occur after rehydrating the IPG strips for 1st dimension?

I have been facing problem while rehydration of the IPG strips with samples. Some sort of sediments are present below the strip which stick to the plate. Moreover the surface of the strips seems...

01 April 2016 257 0 View