Abhinav Prasad

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Questions related from Abhinav Prasad

Why low sensitivity of MTT test for determining cell viability in fish cell lines?

While performing cell viability assay using MTT in fish cell lines grown in L-15 media without CO2 at 28 degrees, I found a highly faint purple colour in control cells (after solubilising formazan...

21 March 2020 8,257 3 View

Fold MFI vs % change in + and - cells, which one is better way of representing a histogram for a fluorescent event measured by flow cytometry?Why?

Generally flow cytometric fluorescence data are reported in two ways on a 2D-histogram as indicated in the attached fig. Fold change or % change or change(by just plotting values obtained by FACS...

24 December 2019 9,426 4 View

What are the differences among different MFIs(M=Median,Geometric mean and Mean(Arithmetic) Which one to use when and why?

For plotting and analysing fluorescence intensities of control and treated samples on a flow cytometry histogram, different MFIs (Median, Geometric mean and Arithmetic mean) have been used by...

24 December 2019 4,224 3 View

What pecentage of starting concentration of protein (say100ug) is lost at each acetone precipitation step before iTRAQ labeling of peptides?

what should be the concentration of proteins to start with if one has to label 100ug of proteins with iTRAQ labels since some amount of proteins would be lost after acetone precipitation(during...

19 June 2015 1,952 2 View

How do I isolate mitochondria from cultured murine fibroblast cells?

I have tried isolating mitochondria from fibroblasts many times using different protocols like C Frezaa,2007;E Gottlieb,2003;Wieckowski,2009;B Antonsson,2000 etc(which slightiy differs from one...

16 May 2015 6,440 2 View

Journal Impact Factor 2020 (Clarivate Analytics Journal Citation Reports 2021) and its impact on Journal's competitiveness/article acceptance rate

JCR, 2021 has now been released. Journal Impact factor, its Ranking and Quartile in a category can be viewed at the following link by writing the journal's name in "basic search" and selecting...

01 January 1970 6,935 6 View