Abhilasha Singh

3 Questions 6 Answers 0 Followers

Questions related from Abhilasha Singh

Why am I getting the same sequence from both the forward and reverse primers in Sanger sequencing?

I have cloned a 2.7 kb insert into the pCAMBIA1300 vector and confirmed the cloning by restriction digestion and plasmid PCR. However, after Sanger sequencing with M13F and M13R primers, I am...

13 March 2025 958 7 View

Can I clone an RCA product into the TA vector?

I was trying to clone an RCA product (digested with a single restriction endonuclease) into the pUC 19 vector but I failed. After cloning, I have to go for the sequencing. Can I clone it into a TA...

25 October 2024 3,906 5 View

Why am I not getting any positive colonies in my transformed GV3101 Plate?

I had transformed positive plasmid in GV3101 and got colonies after 2 days. But I did not get a single positive colony after colony PCR. What will be the reason? Although I used required...

28 May 2024 5,626 0 View