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Questions related from Abhay Kanodia
Hello all, I am over-expressing acid sphingomyelinase enzyme in HeLa cells. Theenzyme shows expression on western blot but I do not have any activity in in vitro assays. I have checked the...
07 July 2023 341 3 View
Hello, I am using the following tutorial for microRNA profiling: https://drmirdeep.github.io/mirdeep2_tutorial.html I was able to follow it until the patching step which is: bash...
01 November 2022 6,663 0 View
Does anyone have experience growing single cell clones of MCF-10A.I have generated gene knockouts and need to pick single cell clones from these cell lines. It is challenging as these cells are...
29 April 2021 1,815 2 View
I am using MCF10A cell lines. I see different sensitivity to Blasticidin of my control cells (not infected with lentivirus) vs cells infected with lentivirus. I thought this is a good thing as My...
26 February 2021 7,038 1 View
I am making CRISPR knockouts in MCF10A cells and Blasticidin is used as a selection marker. When I add Blasticidin, some cells die which is expected as they haven't received my sgRNA. However,...
12 December 2020 4,692 2 View
I digested my lenti guide puro plasmid. The only modification in this plasmid is that it has blasticidin resistance gene instead of puromycin. I get the desired bands at ~8kb but I do not see the...
04 August 2020 2,956 4 View
I have added anti- CD28/anti-CD3 beads to in vitro culture of murine T cells. Some of the cells have increased in size which indicates their activation. However, is there a method to quantify the...
09 August 2019 3,502 7 View
Hi, I have attached images for the two questions I have. I use SYBR green for quantification. (a) I have my gene NF2 and I get a different melt curve for different samples. I get a single peak...
01 January 1970 9,264 2 View
