Abhay Kanodia

3 Questions 1 Answers 0 Followers

Questions related from Abhay Kanodia

Does anyone have experience with Blasticidin selection after lentiviral transduction?

I am using MCF10A cell lines. I see different sensitivity to Blasticidin of my control cells (not infected with lentivirus) vs cells infected with lentivirus. I thought this is a good thing as My...

26 February 2021 7,033 1 View

Why do I get a lot of colonies upon transformation of digested lenti guide puro?

I digested my lenti guide puro plasmid. The only modification in this plasmid is that it has blasticidin resistance gene instead of puromycin. I get the desired bands at ~8kb but I do not see the...

04 August 2020 2,934 4 View

QPCR- (a) Why do I have different melt curves for same primer (b) gene of interest value lower than GAPDH?

Hi, I have attached images for the two questions I have. I use SYBR green for quantification. (a) I have my gene NF2 and I get a different melt curve for different samples. I get a single peak...

01 January 1970 9,257 2 View